This toolkit has been developed to assist facility level administrators and health care workers in communities with a high prevalence of both TB and HIV to implement TB infection prevention and control measures, using the “Three I’s” framework as articulated by the World Health Organization. The toolkit provides step-by-step guidance on strengthening TB infection control practices at the facility, intensifying case finding among HIV-infected patients, and incorporating isoniazid preventive therapy into the care and treatment provided to persons living with HIV.